GLO1 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5131R-FITC |
| Product Name | GLO1 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GLO1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2739 |
| Subcellular location | Cytoplasm, Nucleus, Secreted, Cell membrane |
| Synonyms | Aldoketomutase; GLOD1; Glx I; GLYI; Glyoxalase I; Ketone aldehyde mutase; Lactoyl glutathione lyase; S D lactoylglutathione methylglyoxal lyase; LGUL_HUMAN; glo1; glyoxalase domain containing 1; Ketone-aldehyde mutase; Methylglyoxalase. |
| Background | The enzyme encoded by GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GLO1 Polyclonal Antibody, FITC Conjugated (bs-5131R-FITC) validated for, and at what dilution?
bs-5131R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GLO1 Polyclonal Antibody, FITC Conjugated (bs-5131R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, GuineaPig.
How should bs-5131R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GLO1 Polyclonal Antibody, FITC Conjugated (bs-5131R-FITC)?
KLH conjugated synthetic peptide derived from human GLO1
What are the excitation and emission wavelengths of the FITC conjugate (bs-5131R-FITC)?
FITC on bs-5131R-FITC: excitation / emission 494nm/518nm.