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GLO1 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • GuineaPig
Overview
Catalog # bs-5131R-HRP
Product Name GLO1 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse, GuineaPig
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GLO1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2739
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms Aldoketomutase; GLOD1; Glx I; GLYI; Glyoxalase I; Ketone aldehyde mutase; Lactoyl glutathione lyase; S D lactoylglutathione methylglyoxal lyase; LGUL_HUMAN; glo1; glyoxalase domain containing 1; Ketone-aldehyde mutase; Methylglyoxalase.
Background The enzyme encoded by GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is GLO1 Polyclonal Antibody, HRP Conjugated (bs-5131R-HRP) validated for, and at what dilution?

bs-5131R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GLO1 Polyclonal Antibody, HRP Conjugated (bs-5131R-HRP) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, GuineaPig.

How should bs-5131R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GLO1 Polyclonal Antibody, HRP Conjugated (bs-5131R-HRP)?

KLH conjugated synthetic peptide derived from human GLO1