CREM Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5135R-Cy7 |
| Product Name | CREM Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CREM |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1390 |
| Swiss Prot | Q03060 |
| Subcellular location | Nucleus |
| Synonyms | cAMP response element modulator; CREM-1; cAMP responsive element modulator; hCREM 2; hCREM2; ICER; Inducible cAMP early repressor; Inducible cAMP early repressor ICER; MGC111110; MGC17881; MGC41893. |
| Background | CREM is a a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is derived from a multiexonic gene that encodes both activators and antagonists of cAMP-inducible transcription by differential splicing. Splice variants with antagonistic function lack 2 glutamine-rich domains and block cAMP-induced transcription, whereas an isoform that includes these glutamine-rich domains is a transcriptional activator. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CREM Polyclonal Antibody, Cy7 Conjugated (bs-5135R-Cy7) validated for, and at what dilution?
bs-5135R-Cy7 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CREM Polyclonal Antibody, Cy7 Conjugated (bs-5135R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Chicken.
How should bs-5135R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CREM Polyclonal Antibody, Cy7 Conjugated (bs-5135R-Cy7)?
KLH conjugated synthetic peptide derived from human CREM
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5135R-Cy7)?
Cy7 on bs-5135R-Cy7: excitation / emission 743nm/767nm.