TAF12 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5142R-RBITC-100uL |
| Product Name | TAF12 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TAF12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6883 |
| Subcellular location | Nucleus |
| Synonyms | TAF12 RNA polymerase II, TATA box binding protein TBP-associated factor, 20kDa; TAF15; TAF2J; TAFII 20/TAFII 15; TAFII20; TAFII20/TAFII15; Transcription initiation factor TFIID 20/15 kDa subunits; Transcription initiation factor TFIID subunit 12; TAF12_HUMAN. |
| Background | TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP free TAFII complex (TFTC). TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TAF12 Polyclonal Antibody, RBITC Conjugated (bs-5142R-RBITC) validated for, and at what dilution?
bs-5142R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TAF12 Polyclonal Antibody, RBITC Conjugated (bs-5142R-RBITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Chicken.
How should bs-5142R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TAF12 Polyclonal Antibody, RBITC Conjugated (bs-5142R-RBITC)?
KLH conjugated synthetic peptide derived from human TAF12
What are the excitation and emission wavelengths of the RBITC conjugate (bs-5142R-RBITC)?
RBITC on bs-5142R-RBITC: excitation / emission 540nm/625nm.