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AKT3 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Cerebrum lysates; Lane 2: Testis lysates probed with AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
Lane 1: Cerebrum lysates; Lane 2: Testis lysates probed with AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-5146R
Product Name AKT3 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AKT3
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 207
Swiss Prot P31749
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms AKT3 kinase; DKFZP434N0250; PKB Gamma; PKBG; PRKBG; Protein Kinase AKT3; Protein Kinase B Gamma; RAC gamma; RAC Gamma Serine/Threonine Protein Kinase; RACPK Gamma; Serine threonine protein kinase Akt 3; Serine Threonine Protein Kinase AKT3; STK 2; STK2; V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma; VAKT Murine Thymoma Viral Oncogene Homolog 3; AKT3_HUMAN.
Application Dilution
WB =1:500-2000
IHC-P ELISAELISA=1:5000-10000
IHC-F
IF

Frequently asked questions

Which applications is AKT3 Polyclonal Antibody (bs-5146R) validated for, and at what dilution?

bs-5146R is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-10000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AKT3 Polyclonal Antibody (bs-5146R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Horse, Chicken, Rabbit.

How should bs-5146R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AKT3 Polyclonal Antibody (bs-5146R)?

KLH conjugated synthetic peptide derived from human AKT3

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