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Acrosin Polyclonal Antibody, PE-Cy7 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-5151R-PE-Cy7-100uL
Product Name Acrosin Polyclonal Antibody, PE-Cy7 Conjugated
Applications
WB FCM
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation PE-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Acrosin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 49
Swiss Prot P10323
Subcellular location Cytoplasm, Nucleus
Synonyms Acrosin; ACR; ACRS
Background Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is Acrosin Polyclonal Antibody, PE-Cy7 Conjugated (bs-5151R-PE-Cy7) validated for, and at what dilution?

bs-5151R-PE-Cy7 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Acrosin Polyclonal Antibody, PE-Cy7 Conjugated (bs-5151R-PE-Cy7) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-5151R-PE-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Acrosin Polyclonal Antibody, PE-Cy7 Conjugated (bs-5151R-PE-Cy7)?

KLH conjugated synthetic peptide derived from human Acrosin

What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-5151R-PE-Cy7)?

PE-Cy7 on bs-5151R-PE-Cy7: excitation / emission 488nm/780nm.