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phospho-LSP1 (Ser204) Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Horse
  • Chicken
Overview
Catalog # bs-5155R-Biotin
Product Name phospho-LSP1 (Ser204) Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Specificity This phosphorylation site is homologous to that of Ser195 in Mouse, Ser196 in Rat and Ser228 in Bovine.
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Horse, Chicken
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human LSP1 around the phosphorylation site of Ser204
Modification Site Ser204
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 4046
Swiss Prot P33241
Subcellular location Cytoplasm, Cell membrane
Synonyms WP34; pp52; Lymphocyte-specific protein 1; 47 kDa actin-binding protein; 52 kDa phosphoprotein; Lymphocyte-specific antigen WP34; LSP1
Background May play a role in mediating neutrophil activation and chemotaxis. This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is phospho-LSP1 (Ser204) Polyclonal Antibody, Biotin Conjugated (bs-5155R-Biotin) validated for, and at what dilution?

bs-5155R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-LSP1 (Ser204) Polyclonal Antibody, Biotin Conjugated (bs-5155R-Biotin) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Horse, Chicken.

How should bs-5155R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-LSP1 (Ser204) Polyclonal Antibody, Biotin Conjugated (bs-5155R-Biotin)?

KLH conjugated synthetic phosphopeptide derived from human LSP1 around the phosphorylation site of Ser204