phospho-LSP1 (Ser204) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5155R-Cy3 |
| Product Name | phospho-LSP1 (Ser204) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Ser195 in Mouse, Ser196 in Rat and Ser228 in Bovine. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human LSP1 around the phosphorylation site of Ser204 |
| Modification Site | Ser204 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4046 |
| Swiss Prot | P33241 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | WP34; pp52; Lymphocyte-specific protein 1; 47 kDa actin-binding protein; 52 kDa phosphoprotein; Lymphocyte-specific antigen WP34; LSP1 |
| Background | May play a role in mediating neutrophil activation and chemotaxis. This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-LSP1 (Ser204) Polyclonal Antibody, Cy3 Conjugated (bs-5155R-Cy3) validated for, and at what dilution?
bs-5155R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-LSP1 (Ser204) Polyclonal Antibody, Cy3 Conjugated (bs-5155R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Horse, Chicken.
How should bs-5155R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-LSP1 (Ser204) Polyclonal Antibody, Cy3 Conjugated (bs-5155R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human LSP1 around the phosphorylation site of Ser204
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5155R-Cy3)?
Cy3 on bs-5155R-Cy3: excitation / emission 512,550nm/570,615nm.