TDP2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5164R-Cy5 |
| Product Name | TDP2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ETS1 associated protein II/EAPII |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | EAP 2; EAP II; EAP2; EAPII; ETS 1 associated protein 2; ETS 1 associated protein II; ETS1 associated protein 2; ETS1-associated protein 2; ETS1-associated protein II; tdp2; TRAF and TNF receptor associated protein; TRAF and TNF receptor-associated protein; TTRAP; TYDP2_HUMAN; Tyr DNA phosphodiesterase 2; Tyr-DNA phosphodiesterase 2; Tyrosyl DNA phosphodiesterase 2; Tyrosyl-DNA phosphodiesterase 2; 5''-Tyr-DNA phosphodiesterase; 5''-tyrosyl-DNA phosphodiesterase; AD 022; AD022; MGC111021; MGC9099. |
| Background | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TDP2 Polyclonal Antibody, Cy5 Conjugated (bs-5164R-Cy5) validated for, and at what dilution?
bs-5164R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TDP2 Polyclonal Antibody, Cy5 Conjugated (bs-5164R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-5164R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TDP2 Polyclonal Antibody, Cy5 Conjugated (bs-5164R-Cy5)?
KLH conjugated synthetic peptide derived from human ETS1 associated protein II/EAPII
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5164R-Cy5)?
Cy5 on bs-5164R-Cy5: excitation / emission 625,650nm/670nm.