phospho-APBB1 (Ser175) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5165R-Cy3 |
| Product Name | phospho-APBB1 (Ser175) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human APBB1 around the phosphorylation site of Ser175 [LS(p-S)PP] |
| Modification Site | Ser175 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 322 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Adaptor protein FE65a2; Amyloid beta A4 precursor protein binding family B member 1; Amyloid beta A4 precursor protein binding family B; Amyloid beta A4 precursor protein binding family B member 1; Amyloid beta precursor protein binding family B member 1; APBB 1; APBB1; FE 65; Fe65 protein; MGC 9072; MGC9072; Protein Fe65; RIR; Stat like protein; Fe-65 Protein; APBB1_HUMAN. |
| Background | The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-APBB1 (Ser175) Polyclonal Antibody, Cy3 Conjugated (bs-5165R-Cy3) validated for, and at what dilution?
bs-5165R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-APBB1 (Ser175) Polyclonal Antibody, Cy3 Conjugated (bs-5165R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-5165R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-APBB1 (Ser175) Polyclonal Antibody, Cy3 Conjugated (bs-5165R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human APBB1 around the phosphorylation site of Ser175 [LS(p-S)PP]
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5165R-Cy3)?
Cy3 on bs-5165R-Cy3: excitation / emission 512,550nm/570,615nm.