phospho-Amyloid Precursor Protein (Ser730) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5168R-BF405-100uL |
| Product Name | phospho-Amyloid Precursor Protein (Ser730) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human APP770 around the phosphorylation site of Ser730 |
| Modification Site | Ser730 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 351 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane, Extracellular matrix |
| Synonyms | Amyloid Precursor Protein phospho S730; Amyloid Precursor Protein phospho Ser730; p-Amyloid Precursor Protein phospho S730; APP Ser730; p-APPSer730; C31; C80; C83; C99; beta-amyloid precursor protein C-terminus; A4 amyloid protein; A4; AAA; ABETA; ABPP; AD 1; AD1; Alzheimer disease 1; Alzheimer disease; Alzheimer's disease amyloid protein; Amyloid beta A4 precursor protein; Amyloid beta A4 protein; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform a; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta protein; Amyloid beta-peptide; Amyloid of aging and alzheimer disease; APP; APP I ; APPI; Beta amyloid peptide; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Human mRNA for amyloid A4 precursor of Alzheimer's disease; PN 2; PN II; PN2; PreA4; Protease nexin II; A4_HUMAN. |
| Background | This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Amyloid Precursor Protein (Ser730) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5168R-BF405) validated for, and at what dilution?
bs-5168R-BF405 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Amyloid Precursor Protein (Ser730) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5168R-BF405) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5168R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Amyloid Precursor Protein (Ser730) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5168R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human APP770 around the phosphorylation site of Ser730
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-5168R-BF405)?
AbBy Fluor-405 on bs-5168R-BF405: excitation / emission 401nm/421nm.