phospho-ATF2 (Ser322) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5169R-Cy5 |
| Product Name | phospho-ATF2 (Ser322) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser322 |
| Modification Site | Ser322 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11909 |
| Subcellular location | Nucleus |
| Synonyms | ATF2 phospho Ser322; ATF2 phospho S322; p-ATF2 S322; p-ATF2 Ser322; CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_MOUSE. |
| Background | ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ATF2 (Ser322) Polyclonal Antibody, Cy5 Conjugated (bs-5169R-Cy5) validated for, and at what dilution?
bs-5169R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ATF2 (Ser322) Polyclonal Antibody, Cy5 Conjugated (bs-5169R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Horse, Rabbit.
How should bs-5169R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ATF2 (Ser322) Polyclonal Antibody, Cy5 Conjugated (bs-5169R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser322
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5169R-Cy5)?
Cy5 on bs-5169R-Cy5: excitation / emission 625,650nm/670nm.