phospho-APC1 (Ser377) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5174R-Cy3 |
| Product Name | phospho-APC1 (Ser377) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ANAPC1 around the phosphorylation site of Ser377 |
| Modification Site | Ser377 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 64682 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Apc1 phospho S377; p-Apc1 phospho S377; ANAPC1Ser377; ANAPC 1; ANAPC1; Anaphase promoting complex subunit 1; anaphase-promoting complex 1 meiotic checkpoint regulator; Anaphase-promoting complex subunit 1; Apc 1; APC1; APC1_HUMAN; Cyclosome subunit 1; MCPR; Meiotic checkpoint regulator; Mitotic checkpoint regulator; Protein Tsg 24; Protein Tsg24; Testis-specic gene 24 protein; TSG 24; TSG24. |
| Background | This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-APC1 (Ser377) Polyclonal Antibody, Cy3 Conjugated (bs-5174R-Cy3) validated for, and at what dilution?
bs-5174R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-APC1 (Ser377) Polyclonal Antibody, Cy3 Conjugated (bs-5174R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-5174R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-APC1 (Ser377) Polyclonal Antibody, Cy3 Conjugated (bs-5174R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human ANAPC1 around the phosphorylation site of Ser377
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5174R-Cy3)?
Cy3 on bs-5174R-Cy3: excitation / emission 512,550nm/570,615nm.