phospho-PTPRA (Ser180) Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5175R-Cy3 |
| Product Name | phospho-PTPRA (Ser180) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PTPRA around the phosphorylation site of Ser180 |
| Modification Site | Ser180 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5786 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | PTPRA Ser180; PTPRA S180; p-PTPRA Ser180; p-PTPRA S180; HEPTP; HLPR; HPTP alpha; HPTPA; HPTPalpha; Leukocyte common antigen related peptide; LRP; Protein tyrosine phosphatase alpha; Protein tyrosine phosphatase receptor type A; Protein tyrosine phosphatase receptor type alpha polypeptide; Protein tyrosine phosphate; Protein-tyrosine phosphatase alpha; PTPA; PTPalpha; PTPase alpha; PTPLCA related phosphatase; PTPRA; PTPRA_HUMAN; PTPRL 2; PTPRL2; PTRA; R PTP alpha; R-PTP-alpha; Receptor type tyrosine protein phosphatase alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTPA; Tyrosine phosphatase alpha. |
| Background | Vital cellular functions such as cell proliferation and signal transduction are regulated in part by the balance between the activities of protein tyrosine kinases (PTK) and protein tyrosine phosphatases (PTPs). The protein contains a 121 residue extracellular domain, a single transmembrane segment, and 2 tandem intracytoplasmic catalytic domains. The PTP alpha protein is ubiquitously expressed and may play a fundamental role in the physiology of all cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PTPRA (Ser180) Polyclonal Antibody, Cy3 Conjugated (bs-5175R-Cy3) validated for, and at what dilution?
bs-5175R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5175R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PTPRA (Ser180) Polyclonal Antibody, Cy3 Conjugated (bs-5175R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human PTPRA around the phosphorylation site of Ser180
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5175R-Cy3)?
Cy3 on bs-5175R-Cy3: excitation / emission 512,550nm/570,615nm.