phospho-ATF4 (Ser245) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5190R-APC-Cy7-100uL |
| Product Name | phospho-ATF4 (Ser245) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ATF4 around the phosphorylation site of Ser245 |
| Modification Site | Ser245 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 468 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | ATF4 phospho S245; ATF4Ser245; Cyclic AMP response element binding protein 2; DNA binding protein TAXREB67; Activating Transcription Factor 4; ATF 4; ATF4 protein; CREB 2; CREB2; CREB-2; Cyclic AMP dependent transcription factor ATF 4; Tax Responsive Enhancer Element B67; TAXREB67; TXREB. |
| Background | ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ATF4 (Ser245) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5190R-APC-Cy7) validated for, and at what dilution?
bs-5190R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ATF4 (Ser245) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5190R-APC-Cy7) react with?
Tested reactivity: Human.
How should bs-5190R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ATF4 (Ser245) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5190R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human ATF4 around the phosphorylation site of Ser245
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-5190R-APC-Cy7)?
APC-Cy7 on bs-5190R-APC-Cy7: excitation / emission 650nm/780nm.