phospho-AR/Androgen receptor (Ser213) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5191R-Biotin |
| Product Name | phospho-AR/Androgen receptor (Ser213) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser213 [ER(p-S)GA] |
| Modification Site | Ser213 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 367 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Androgen Receptor phospho S213; Androgen Receptor Ser213; Androgen Receptor phospho Ser213; p-Androgen Receptor Ser213; ANDR_HUMAN; HYSP1; AIS; Androgen receptor dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease; AR; DHTR; Dihydro Testosterone Receptor; Dihydrotestosterone receptor; HUMARA; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; Spinal and bulbar muscular atrophy; TFM. |
| Background | The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-AR/Androgen receptor (Ser213) Polyclonal Antibody, Biotin Conjugated (bs-5191R-Biotin) validated for, and at what dilution?
bs-5191R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-AR/Androgen receptor (Ser213) Polyclonal Antibody, Biotin Conjugated (bs-5191R-Biotin) react with?
Tested reactivity: Human.
How should bs-5191R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-AR/Androgen receptor (Ser213) Polyclonal Antibody, Biotin Conjugated (bs-5191R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser213 [ER(p-S)GA]