phospho-ATG9A (Ser735) Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5199R-RBITC-100uL |
| Product Name | phospho-ATG9A (Ser735) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ATG9A around the phosphorylation site of Ser735 |
| Modification Site | Ser735 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79065 |
| Swiss Prot | Q7Z3C6 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | mATG9; APG9L1; MGD3208; Autophagy-related protein 9A; APG9-like 1; ATG9A |
| Background | Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ATG9A (Ser735) Polyclonal Antibody, RBITC Conjugated (bs-5199R-RBITC) validated for, and at what dilution?
bs-5199R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5199R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ATG9A (Ser735) Polyclonal Antibody, RBITC Conjugated (bs-5199R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human ATG9A around the phosphorylation site of Ser735
What are the excitation and emission wavelengths of the RBITC conjugate (bs-5199R-RBITC)?
RBITC on bs-5199R-RBITC: excitation / emission 540nm/625nm.