phospho-ATG4D (Ser467) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5203R-BF405-100uL |
| Product Name | phospho-ATG4D (Ser467) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated syntheticphosphopeptide derived from human ATG4D around the phosphorylation site of Ser467 [RP(p-S)SE] |
| Modification Site | Ser467 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84971 |
| Subcellular location | Cytoplasm |
| Synonyms | APG4 D; APG4D; ATG4 autophagy related 4 homolog D; Atg4dl; AUT like 4 cysteine endopeptidase; AUTL4; Autophagin 4; Autophagy related cysteine endopeptidase 4; Autophagy related protein 4 homolog D; Cysteine protease ATG4D. |
| Background | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ATG4D (Ser467) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5203R-BF405) validated for, and at what dilution?
bs-5203R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ATG4D (Ser467) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5203R-BF405) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-5203R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ATG4D (Ser467) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5203R-BF405)?
KLH conjugated syntheticphosphopeptide derived from human ATG4D around the phosphorylation site of Ser467 [RP(p-S)SE]
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-5203R-BF405)?
AbBy Fluor-405 on bs-5203R-BF405: excitation / emission 401nm/421nm.