phospho-ATG4D (Ser467) Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-ATG4D(Ser467) Polyclonal Antibody, Unconjugated (bs-5203R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-ATG4D(Ser467) Polyclonal Antibody, Unconjugated (bs-5203R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Lung lysates; Lane 4: Mouse Testis lysates; Lane 5: Mouse Uterus lysates probed with ATG4D Polyclonal Antibody, Unconjugated (bs-5203R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-5203R
Product Name	phospho-ATG4D (Ser467) Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated syntheticphosphopeptide derived from human ATG4D around the phosphorylation site of Ser467 [RP(p-S)SE]
Modification Site	Ser467
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84971
Subcellular location	Cytoplasm
Synonyms	APG4 D; APG4D; ATG4 autophagy related 4 homolog D; Atg4dl; AUT like 4 cysteine endopeptidase; AUTL4; Autophagin 4; Autophagy related cysteine endopeptidase 4; Autophagy related protein 4 homolog D; Cysteine protease ATG4D.
Background	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200