Bad (Ser118) Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-5216R-PE
Product Name Bad (Ser118) Polyclonal Antibody, PE Conjugated
Applications WB, FCM
Specificity KO-Validated
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser118
Modification Site Ser118
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 572
Swiss Prot Q92934
Subcellular location Cytoplasm, Cell membrane
Synonyms Bad phospho S118; Bad phospho Ser118; p-Bad S118;p- Bad Ser118; p-Bad phospho Ser118; BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; mouse BAD Ser155, rat BAD Ser156
Background Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Application Dilution
WB 1:300-5000
FCM 1:20-100