phospho-BRAF (Thr401) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5224R-FITC |
| Product Name | phospho-BRAF (Thr401) Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Thr401 |
| Modification Site | Thr401 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 673 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | B Raf phospho T401; p-B Raf phospho T401; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF 1; Braf; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral v-raf oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; p94; RAFB 1; RAFB1; v raf murine sarcoma viral oncogene homolog B1; FLJ95109; BRAF_HUMAN. |
| Background | The Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-BRAF (Thr401) Polyclonal Antibody, FITC Conjugated (bs-5224R-FITC) validated for, and at what dilution?
bs-5224R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BRAF (Thr401) Polyclonal Antibody, FITC Conjugated (bs-5224R-FITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5224R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BRAF (Thr401) Polyclonal Antibody, FITC Conjugated (bs-5224R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Thr401
What are the excitation and emission wavelengths of the FITC conjugate (bs-5224R-FITC)?
FITC on bs-5224R-FITC: excitation / emission 494nm/518nm.