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Cardiodilatin-related peptide Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
Overview
Catalog # bs-5225R-Cy3
Product Name Cardiodilatin-related peptide Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cardiodilatin-related peptide:26-55/153
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4878
Swiss Prot P01160
Subcellular location Secreted
Synonyms Prepronatriodilatin; ANF_HUMAN; ANF; Atrial natriuretic factor; Cardiodilatin related peptide; CDD ANF; CDP; PND; Prepronatriodilatin; Nppa; Natriuretic peptides A; Atrial natriuretic peptide; CDD-ANF; Cardiodilatin-related peptide; ANP.
Background Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Cardiodilatin-related peptide Polyclonal Antibody, Cy3 Conjugated (bs-5225R-Cy3) validated for, and at what dilution?

bs-5225R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cardiodilatin-related peptide Polyclonal Antibody, Cy3 Conjugated (bs-5225R-Cy3) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog.

How should bs-5225R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cardiodilatin-related peptide Polyclonal Antibody, Cy3 Conjugated (bs-5225R-Cy3)?

KLH conjugated synthetic peptide derived from human Cardiodilatin-related peptide:26-55/153

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5225R-Cy3)?

Cy3 on bs-5225R-Cy3: excitation / emission 512,550nm/570,615nm.