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phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody

Formalin-fixed and human breast carcinoma tissue labeled with Anti-phospho-BACH1\/BRIP1(Ser990) Polyclonal Antibody, Unconjugated (bs-5226R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and human breast carcinoma tissue labeled with Anti-phospho-BACH1\/BRIP1(Ser990) Polyclonal Antibody, Unconjugated (bs-5226R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-BRIP1\/BACH1 (Ser990) Polyclonal \r\nAntibody(bs-5226R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-BRIP1\/BACH1 (Ser990) Polyclonal \r\nAntibody(bs-5226R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Cow
  • Sheep
Overview
Catalog # bs-5226R
Product Name phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This phosphorylation site is homologous to that of Ser921 in Bovine.
Reactivity Human, Mouse
Predicted Reactivity Cow, Sheep
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human BRIP1 around the phosphorylation site of Ser990
Modification Site Ser990
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 83990
Swiss Prot Q9BX63
Subcellular location Nucleus
Synonyms OF; BACH1; FANCJ; Fanconi anemia group J protein; Protein FACJ; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1; BRIP1
Background DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody (bs-5226R) validated for, and at what dilution?

bs-5226R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody (bs-5226R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.

How should bs-5226R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody (bs-5226R)?

KLH conjugated synthetic phosphopeptide derived from human BRIP1 around the phosphorylation site of Ser990

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