phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5226R-Cy5 |
| Product Name | phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Ser921 in Bovine. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Cow, Sheep |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human BRIP1 around the phosphorylation site of Ser990 |
| Modification Site | Ser990 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83990 |
| Swiss Prot | Q9BX63 |
| Subcellular location | Nucleus |
| Synonyms | OF; BACH1; FANCJ; Fanconi anemia group J protein; Protein FACJ; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1; BRIP1 |
| Background | DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, Cy5 Conjugated (bs-5226R-Cy5) validated for, and at what dilution?
bs-5226R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, Cy5 Conjugated (bs-5226R-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.
How should bs-5226R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, Cy5 Conjugated (bs-5226R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human BRIP1 around the phosphorylation site of Ser990
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5226R-Cy5)?
Cy5 on bs-5226R-Cy5: excitation / emission 625,650nm/670nm.