phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5226R-FITC |
| Product Name | phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Ser921 in Bovine. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Cow, Sheep |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human BRIP1 around the phosphorylation site of Ser990 |
| Modification Site | Ser990 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83990 |
| Swiss Prot | Q9BX63 |
| Subcellular location | Nucleus |
| Synonyms | OF; BACH1; FANCJ; Fanconi anemia group J protein; Protein FACJ; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1; BRIP1 |
| Background | DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, FITC Conjugated (bs-5226R-FITC) validated for, and at what dilution?
bs-5226R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, FITC Conjugated (bs-5226R-FITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.
How should bs-5226R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BRIP1/BACH1 (Ser990) Polyclonal Antibody, FITC Conjugated (bs-5226R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human BRIP1 around the phosphorylation site of Ser990
What are the excitation and emission wavelengths of the FITC conjugate (bs-5226R-FITC)?
FITC on bs-5226R-FITC: excitation / emission 494nm/518nm.