phospho-CDKN1A/p21 (Thr145) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5237R-APC-100uL |
| Product Name | phospho-CDKN1A/p21 (Thr145) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Thr140 in Mouse and Thr145 is Rat. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr145 [RQ(p-T)S] |
| Modification Site | Thr145 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1026 |
| Swiss Prot | P38936 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; CDKN1A; MDA6; PIC1 |
| Background | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CDKN1A/p21 (Thr145) Polyclonal Antibody, APC Conjugated (bs-5237R-APC) validated for, and at what dilution?
bs-5237R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CDKN1A/p21 (Thr145) Polyclonal Antibody, APC Conjugated (bs-5237R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-5237R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CDKN1A/p21 (Thr145) Polyclonal Antibody, APC Conjugated (bs-5237R-APC)?
KLH conjugated synthetic phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr145 [RQ(p-T)S]
What are the excitation and emission wavelengths of the APC conjugate (bs-5237R-APC)?
APC on bs-5237R-APC: excitation / emission 650nm/660nm.