phospho-Caspase 6 (Ser257) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5240R-Cy5 |
| Product Name | phospho-Caspase 6 (Ser257) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Caspase 6 around the phosphorylation site of Ser257 |
| Modification Site | Ser257 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 839 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Apoptosis-related cysteine protein; Apoptosis Related Cysteine Protease; Apoptotic cysteine protease MCH2; Apoptotic protease MCH 2; Apoptotic protease MCH2; Casp 6; Casp6; Caspase 6 apoptosis related cysteine protease; Caspase 6 precursor; Caspase6; Human cysteine protease Mch2 isoform alpha; Mch 2; Mch2; CASP6_HUMAN. |
| Background | Caspase-6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Subunit is compose of heterodimer of a18 kDa (p18) and a 11 kDa (p11) subunit. Subcellular location at cytoplasmic. Cleavages by CPP32, caspase-8 or -10 generate the two active subunits. It belongs to the peptidase C14 family. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Caspase 6 (Ser257) Polyclonal Antibody, Cy5 Conjugated (bs-5240R-Cy5) validated for, and at what dilution?
bs-5240R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Caspase 6 (Ser257) Polyclonal Antibody, Cy5 Conjugated (bs-5240R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-5240R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Caspase 6 (Ser257) Polyclonal Antibody, Cy5 Conjugated (bs-5240R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Caspase 6 around the phosphorylation site of Ser257
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5240R-Cy5)?
Cy5 on bs-5240R-Cy5: excitation / emission 625,650nm/670nm.