Phospho-cdc25A (Ser124) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5242R-Cy7 |
| Product Name | Phospho-cdc25A (Ser124) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser124 [SH(p-S)DS] |
| Modification Site | Ser124 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 993 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cdc 25a; CDC25A2; Cell division cycle 25 homolog A S. pombe; Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform bv D9Ertd393ev Dual specicity phosphatase CDC25A; EC 3.1.3.48; M phase inducer phosphatase 1; MGC115549; MPIP1_HUMAN. |
| Background | Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-cdc25A (Ser124) Polyclonal Antibody, Cy7 Conjugated (bs-5242R-Cy7) validated for, and at what dilution?
bs-5242R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-cdc25A (Ser124) Polyclonal Antibody, Cy7 Conjugated (bs-5242R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-5242R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-cdc25A (Ser124) Polyclonal Antibody, Cy7 Conjugated (bs-5242R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser124 [SH(p-S)DS]
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5242R-Cy7)?
Cy7 on bs-5242R-Cy7: excitation / emission 743nm/767nm.