phospho-Cdc25B (Ser353) Polyclonal Antibody

$Antigen: bs-5111P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-5111P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Mouse bone lysates probed with Cdc25B (Ser353) Polyclonal Antibody, Unconjugated (bs-5245R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P

Reactivity

Mouse

Predicted Reactivity

Human
Rat

Overview
Catalog #	bs-5245R
Product Name	phospho-Cdc25B (Ser353) Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Specificity	This phosphorylation site is homologous to that of Ser351 in Mouse and Ser 349 in Rat.
Reactivity	Mouse
Predicted Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353
Modification Site	Ser353
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	994
Swiss Prot	P30305
Subcellular location	Cytoplasm
Synonyms	M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2
Background	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400