phospho-Cdc25B (Ser353) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5245R |
| Product Name | phospho-Cdc25B (Ser353) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
|
| Specificity | This phosphorylation site is homologous to that of Ser351 in Mouse and Ser 349 in Rat. |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353 |
| Modification Site | Ser353 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 994 |
| Swiss Prot | P30305 |
| Subcellular location | Cytoplasm |
| Synonyms | M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2 |
| Background | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is phospho-Cdc25B (Ser353) Polyclonal Antibody (bs-5245R) validated for, and at what dilution?
bs-5245R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Cdc25B (Ser353) Polyclonal Antibody (bs-5245R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-5245R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Cdc25B (Ser353) Polyclonal Antibody (bs-5245R)?
KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353