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phospho-Cdc25B (Ser353) Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
Overview
Catalog # bs-5245R-HRP
Product Name phospho-Cdc25B (Ser353) Polyclonal Antibody, HRP Conjugated
Applications WB, ELISA, IHC-P
Specificity This phosphorylation site is homologous to that of Ser351 in Mouse and Ser 349 in Rat.
Reactivity Mouse
Predicted Reactivity Human, Rat
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353
Modification Site Ser353
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 994
Swiss Prot P30305
Subcellular location Cytoplasm
Synonyms M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2
Background Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400