Phospho-CDK1 (Ser39) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5247R-HRP |
| Product Name | Phospho-CDK1 (Ser39) Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human cdc2 around the phosphorylation site of Ser39 [LE(p-S)EE] |
| Modification Site | Ser39 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 983 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | P34CDC2; Cdc 2; CDK 1; CDK1; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell Divsion Cycle 2 Protein; CDK1_HUMAN. |
| Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Phospho-CDK1 (Ser39) Polyclonal Antibody, HRP Conjugated (bs-5247R-HRP) validated for, and at what dilution?
bs-5247R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CDK1 (Ser39) Polyclonal Antibody, HRP Conjugated (bs-5247R-HRP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-5247R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CDK1 (Ser39) Polyclonal Antibody, HRP Conjugated (bs-5247R-HRP)?
KLH conjugated synthetic phosphopeptide derived from human cdc2 around the phosphorylation site of Ser39 [LE(p-S)EE]