Phospho-CHEK2 (Ser516) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5257R-BF680-100uL |
| Product Name | Phospho-CHEK2 (Ser516) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of ser516 |
| Modification Site | ser516 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11200 |
| Subcellular location | Nucleus |
| Synonyms | Chk2 Ser516; bA444G7; CHK2 checkpoint homolog; CHK2_HUMAN; Serine/threonine-protein kinase Chk2; CDS 1; CDS1; Checkpoint kinase 2; Checkpoint like protein CHK2; Chek 2; Chek2; Chk 2; CHK2 checkpoint homolog S. pombe; CHK2 checkpoint homolog; HuCds 1; HuCds1; LFS 2; LFS2; PP1425; RAD 53; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2. |
| Background | Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, and may also participate in transduction of the DNA damage and replicational stress signals. Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-CHEK2 (Ser516) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-5257R-BF680) validated for, and at what dilution?
bs-5257R-BF680 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CHEK2 (Ser516) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-5257R-BF680) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Chicken, Rabbit.
How should bs-5257R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CHEK2 (Ser516) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-5257R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of ser516
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-5257R-BF680)?
AbBy Fluor-680 on bs-5257R-BF680: excitation / emission 679nm/702nm.