Phospho-Cofilin (Tyr140) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5258R-Cy7 |
| Product Name | Phospho-Cofilin (Tyr140) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Cofilin around the phosphorylation site of Tyr140 |
| Modification Site | Tyr140 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1072 |
| Swiss Prot | P23528 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | CFL; cofilin; HEL-S-15; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1 |
| Background | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Cofilin (Tyr140) Polyclonal Antibody, Cy7 Conjugated (bs-5258R-Cy7) validated for, and at what dilution?
bs-5258R-Cy7 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Cofilin (Tyr140) Polyclonal Antibody, Cy7 Conjugated (bs-5258R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig.
How should bs-5258R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Cofilin (Tyr140) Polyclonal Antibody, Cy7 Conjugated (bs-5258R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human Cofilin around the phosphorylation site of Tyr140
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5258R-Cy7)?
Cy7 on bs-5258R-Cy7: excitation / emission 743nm/767nm.