Phospho-Cofilin (Tyr140) Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-Cofilin\/CFL1(Tyr140)Polyclonal Antibody, Unconjugated (bs-5258R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-Cofilin\/CFL1(Tyr140)Polyclonal Antibody, Unconjugated (bs-5258R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Pig

Overview
Catalog #	bs-5258R
Product Name	Phospho-Cofilin (Tyr140) Polyclonal Antibody
Applications	ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Cofilin around the phosphorylation site of Tyr140
Modification Site	Tyr140
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1072
Swiss Prot	P23528
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	CFL; cofilin; HEL-S-15; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1
Background	Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200