phospho-CDC27 (Thr244) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5268R-HRP |
| Product Name | phospho-CDC27 (Thr244) Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Dog, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CDC27 around the phosphorylation site of Thr244 |
| Modification Site | Thr244 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 996 |
| Subcellular location | Nucleus |
| Synonyms | ANAPC3; Anaphase Promoting Complex 3; Anaphase promoting complex protein 3; Anaphase Promoting Complex Subunit 3; Anaphase-promoting complex subunit 3; APC 3; APC3; APC-3; Cdc 27; Cdc27; Cdc-27; CDC27 homolog; CDC27_HUMAN; CDC27Hs; Cell division cycle 27; Cell division cycle protein 27 homolog; H NUC; H-NUC; HNUC; NUC2 homolog. |
| Background | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-CDC27 (Thr244) Polyclonal Antibody, HRP Conjugated (bs-5268R-HRP) validated for, and at what dilution?
bs-5268R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CDC27 (Thr244) Polyclonal Antibody, HRP Conjugated (bs-5268R-HRP) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Pig, Horse, Rabbit.
How should bs-5268R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CDC27 (Thr244) Polyclonal Antibody, HRP Conjugated (bs-5268R-HRP)?
KLH conjugated synthetic phosphopeptide derived from human CDC27 around the phosphorylation site of Thr244