Phospho-CDK2 (Thr14) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5277R-PE-Cy5-100uL |
| Product Name | Phospho-CDK2 (Thr14) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CDK2 around the phosphorylation site of Thr14 [EG(p-T)YG] |
| Modification Site | Thr14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1017 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | p-CDH2 Thr14; CDH2 Thr14; CDH2 T14; Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; cadherin 2 type 1 N-cadherin neuronal; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; NCAD; Neural Cadherin; Neural Cadherin; CADH2_HUMAN. |
| Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. cdk2 is a cell cycle protein closely related to Cdc2 (cdk1) that has proved useful as a marker of proliferation. cdk2 binds cyclin type A and E proteins and controls progression into S-phase. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-CDK2 (Thr14) Polyclonal Antibody, PE-Cy5 Conjugated (bs-5277R-PE-Cy5) validated for, and at what dilution?
bs-5277R-PE-Cy5 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CDK2 (Thr14) Polyclonal Antibody, PE-Cy5 Conjugated (bs-5277R-PE-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse, Rabbit.
How should bs-5277R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CDK2 (Thr14) Polyclonal Antibody, PE-Cy5 Conjugated (bs-5277R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human CDK2 around the phosphorylation site of Thr14 [EG(p-T)YG]
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-5277R-PE-Cy5)?
PE-Cy5 on bs-5277R-PE-Cy5: excitation / emission 488nm/670nm.