phospho-CASC3 (Tyr181) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5283R-Cy7 |
| Product Name | phospho-CASC3 (Tyr181) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CASC3 around the phosphorylation site of Tyr181 |
| Modification Site | Tyr181 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22794 |
| Subcellular location | Nucleus |
| Synonyms | Barentsz protein; Btz; Cancer susceptibility candidate gene 3 protein; Metastatic lymph node protein 51; MLN 51 protein; MLN51; Protein barentsz; Protein CASC3; Protein MLN 51; CASC3_HUMAN. |
| Background | The multiprotein exon junction complex (EJC) is deposited on mRNAs upstream of exonexon junctions as a consequence of pre-mRNA splicing. In mammalian cells, this complex serves as a key modulator of spliced mRNA metabolism. MLN51 is a nucleocytoplasmic shuttling protein that is overexpressed in breast cancer. The function of MLN51 in mammals remains elusive. Its fly homolog, named barentsz, as well as the proteins mago nashi and tsunagi have been shown to be required for proper oskar mRNA localization to the posterior pole of the oocyte. Magoh and Y14, the human homologs of mago nashi and tsunagi, are core components of the exon junction complex (EJC). The EJC is assembled on spliced mRNAs and plays important roles in post-splicing events including mRNA export, nonsense-mediated mRNA decay, and translation. Human MLN51 is an RNA-binding protein present in ribonucleo-protein complexes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CASC3 (Tyr181) Polyclonal Antibody, Cy7 Conjugated (bs-5283R-Cy7) validated for, and at what dilution?
bs-5283R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CASC3 (Tyr181) Polyclonal Antibody, Cy7 Conjugated (bs-5283R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5283R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CASC3 (Tyr181) Polyclonal Antibody, Cy7 Conjugated (bs-5283R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human CASC3 around the phosphorylation site of Tyr181
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5283R-Cy7)?
Cy7 on bs-5283R-Cy7: excitation / emission 743nm/767nm.