phospho-CD244 (Tyr271) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5284R-Cy5 |
| Product Name | phospho-CD244 (Tyr271) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CD244 around the phosphorylation site of Tyr271 |
| Modification Site | Tyr271 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51744 |
| Swiss Prot | Q9BZW8 |
| Subcellular location | Cell membrane |
| Synonyms | 2B4; NAIL; Nmrk; NKR2B4; SLAMF4; Natural killer cell receptor 2B4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; h2B4; SLAM family member 4; Signaling lymphocytic activation molecule 4; CD244 |
| Background | Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CD244 (Tyr271) Polyclonal Antibody, Cy5 Conjugated (bs-5284R-Cy5) validated for, and at what dilution?
bs-5284R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CD244 (Tyr271) Polyclonal Antibody, Cy5 Conjugated (bs-5284R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Rabbit.
How should bs-5284R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CD244 (Tyr271) Polyclonal Antibody, Cy5 Conjugated (bs-5284R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human CD244 around the phosphorylation site of Tyr271
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5284R-Cy5)?
Cy5 on bs-5284R-Cy5: excitation / emission 625,650nm/670nm.