phospho-CARM1 (Ser228) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5285R-BF405-100uL |
| Product Name | phospho-CARM1 (Ser228) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CARM1 around the phosphorylation site of Ser228 |
| Modification Site | Ser228 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10498 |
| Swiss Prot | Q86X55 |
| Subcellular location | Nucleus |
| Synonyms | CARM1_HUMAN; Coactivator associated arginine methyltransferase 1; Coactivator-associated arginine methyltransferase 1; Histone arginine methyltransferase CARM 1; Histone arginine methyltransferase CARM1; Histone-arginine methyltransferase CARM1; PRMT 4; Protein arginine methyltransferase; Protein arginine N methyltransferase 4; Protein arginine N-methyltransferase 4. |
| Background | Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CARM1 (Ser228) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5285R-BF405) validated for, and at what dilution?
bs-5285R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5285R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CARM1 (Ser228) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5285R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human CARM1 around the phosphorylation site of Ser228
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-5285R-BF405)?
AbBy Fluor-405 on bs-5285R-BF405: excitation / emission 401nm/421nm.