phospho-CDK6 (Tyr24) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5287R-Cy3 |
| Product Name | phospho-CDK6 (Tyr24) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CDK6 around the phosphorylation site of Tyr24 |
| Modification Site | Tyr24 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1021 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CDK6 Tyr24; CDK 6; CDK6; Cell division protein kinase 6; Crk 2; Crk2; Cyclin dependent kinase 6 ; MGC59692; p40; PLSTIRE; Serine/threonine-protein kinase PLSTIRE ; STQTL11; CDK6_HUMAN. |
| Background | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CDK6 (Tyr24) Polyclonal Antibody, Cy3 Conjugated (bs-5287R-Cy3) validated for, and at what dilution?
bs-5287R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CDK6 (Tyr24) Polyclonal Antibody, Cy3 Conjugated (bs-5287R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5287R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CDK6 (Tyr24) Polyclonal Antibody, Cy3 Conjugated (bs-5287R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human CDK6 around the phosphorylation site of Tyr24
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5287R-Cy3)?
Cy3 on bs-5287R-Cy3: excitation / emission 512,550nm/570,615nm.