phospho-DOK1 (Tyr362) Polyclonal Antibody

Lane 1: Jurkat cell lysates; Lane 2: K562 cell lysates probed with DOK1(Tyr362) Polyclonal Antibody, Unconjugated (bs-5289R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig

Overview
Catalog #	bs-5289R
Product Name	phospho-DOK1 (Tyr362) Polyclonal Antibody
Applications	WB, ELISA, FCM
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of Tyr362
Modification Site	Tyr362
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1796
Subcellular location	Cytoplasm
Synonyms	DOK1 phospho Y362; DOK1 phospho Tyr362; p-DOK1 phospho Y362; docking protein 1 downstream of tyrosine kinase 1; Docking protein 1 p62dok Downstream of tyrosine kinase 1 pp62.; docking protein 1 62kD downstream of tyrosine kinase 1; Docking protein 1 62kD; Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK-1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62dok; P62DOK; pp62; pp62.
Background	Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100