phospho-DOK1 (Tyr362) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5289R-APC-100uL |
| Product Name | phospho-DOK1 (Tyr362) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of Tyr362 |
| Modification Site | Tyr362 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1796 |
| Subcellular location | Cytoplasm |
| Synonyms | DOK1 phospho Y362; DOK1 phospho Tyr362; p-DOK1 phospho Y362; docking protein 1 downstream of tyrosine kinase 1; Docking protein 1 p62dok Downstream of tyrosine kinase 1 pp62.; docking protein 1 62kD downstream of tyrosine kinase 1; Docking protein 1 62kD; Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK-1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62dok; P62DOK; pp62; pp62. |
| Background | Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-DOK1 (Tyr362) Polyclonal Antibody, APC Conjugated (bs-5289R-APC) validated for, and at what dilution?
bs-5289R-APC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-DOK1 (Tyr362) Polyclonal Antibody, APC Conjugated (bs-5289R-APC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig.
How should bs-5289R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-DOK1 (Tyr362) Polyclonal Antibody, APC Conjugated (bs-5289R-APC)?
KLH conjugated synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of Tyr362
What are the excitation and emission wavelengths of the APC conjugate (bs-5289R-APC)?
APC on bs-5289R-APC: excitation / emission 650nm/660nm.