phospho-DAXX (Ser495) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5291R-RBITC-100uL |
| Product Name | phospho-DAXX (Ser495) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Ser505 in Bovine. |
| Reactivity | Human |
| Predicted Reactivity | Cow, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human DAXX around the phosphorylation site of Ser495 |
| Modification Site | Ser495 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1616 |
| Swiss Prot | Q9UER7 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DAP6; EAP1; BING2; Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; Fas death domain-associated protein |
| Background | This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-DAXX (Ser495) Polyclonal Antibody, RBITC Conjugated (bs-5291R-RBITC) validated for, and at what dilution?
bs-5291R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-DAXX (Ser495) Polyclonal Antibody, RBITC Conjugated (bs-5291R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig.
How should bs-5291R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-DAXX (Ser495) Polyclonal Antibody, RBITC Conjugated (bs-5291R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human DAXX around the phosphorylation site of Ser495
What are the excitation and emission wavelengths of the RBITC conjugate (bs-5291R-RBITC)?
RBITC on bs-5291R-RBITC: excitation / emission 540nm/625nm.