phospho-Dnmt1 (Tyr399) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5296R-Cy5 |
| Product Name | phospho-Dnmt1 (Tyr399) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Dog, Pig, Horse |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Dnmt1 around the phosphorylation site of Tyr399 |
| Modification Site | Tyr399 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1786 |
| Subcellular location | Nucleus |
| Synonyms | Dnmt1phospho Y399; Dnmt1 phospho Tyr399; p-Dnmt1 Tyr399; AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA cytosine 5 methyltransferase 1; DNA cytosine-5-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase M.HsaI.; DNA MTase; DNA MTase HsaI; DNMT 1; DNMT; Dnmt1; Dnmt1; DNMT1_HUMAN; Dnmt1o; FLJ16293; HSN1E; M.HsaI; MCMT; Met1; MGC104992; mMmul; MommeD2. |
| Background | DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Dnmt1 (Tyr399) Polyclonal Antibody, Cy5 Conjugated (bs-5296R-Cy5) validated for, and at what dilution?
bs-5296R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5296R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Dnmt1 (Tyr399) Polyclonal Antibody, Cy5 Conjugated (bs-5296R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Dnmt1 around the phosphorylation site of Tyr399
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5296R-Cy5)?
Cy5 on bs-5296R-Cy5: excitation / emission 625,650nm/670nm.