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H3K18ac Polyclonal Antibody

To determine the titer, an ELISA was performed using a serial dilution of the H3K18ac antibody (bs-53075R). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,800.
To determine the titer, an ELISA was performed using a serial dilution of the H3K18ac antibody (bs-53075R). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,800.
A Dot Blot analysis was performed to test the cross reactivity of the H3K18ac antibody (bs-53075R) with with peptides containing other modifications and unmodified sequences of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. The figure shows a high specificity of the antibody for the modification of interest.
A Dot Blot analysis was performed to test the cross reactivity of the H3K18ac antibody (bs-53075R) with with peptides containing other modifications and unmodified sequences of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. The figure shows a high specificity of the antibody for the modification of interest.
Bioss unconjugated antibody vial

Applications

  • ELISA
  • dot-blot

Reactivity

  • Human
Overview
Catalog # bs-53075r
Product Name H3K18ac Polyclonal Antibody
Applications
ELISA DOT-BLOT
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against histone H3 containing the acetylated lysine 18 (H3K18ac), using a KLH-conjugated synthetic peptide
Clonality Polyclonal
Isotype Not Determined
Concentration Lot Dependent
Purification Whole antiserum from rabbit containing 0.05% azide
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 8350
Swiss Prot P68431
Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
ELISA 1:500-1000
dot-blot

Frequently asked questions

Which applications is H3K18ac Polyclonal Antibody (bs-53075R) validated for, and at what dilution?

bs-53075R is validated for ELISA (1:500-1000); dot-blot. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does H3K18ac Polyclonal Antibody (bs-53075R) react with?

Tested reactivity: Human.

How should bs-53075R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise H3K18ac Polyclonal Antibody (bs-53075R)?

Polyclonal antibody raised in rabbit against histone H3 containing the acetylated lysine 18 (H3K18ac), using a KLH-conjugated synthetic peptide

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