L3MBTL1 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-53097R-50ug |
| Product Name | L3MBTL1 Polyclonal Antibody |
| Applications |
WB
IP
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Polyclonal antibody raised in rabbit against human L3MBTL1 (lethal 3 malignant brain tumor-like protein), using the N-terminal part of the protein |
| Clonality | Polyclonal |
| Isotype | Not Determined |
| Concentration | 2ug/ul |
| Purification | Whole antiserum from rabbit containing 0.05% azide |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 26013 |
| Swiss Prot | Q9Y468 |
| Synonyms | Lethal(3)malignant brain tumor-like protein 1, L3MBTL1, KIAA0681, L3MBT, L3MBTL |
| Background | Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IF | |
Frequently asked questions
Which applications is L3MBTL1 Polyclonal Antibody (bs-53097R) validated for, and at what dilution?
bs-53097R is validated for WB (1:300-5000); IP (1-2ug); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does L3MBTL1 Polyclonal Antibody (bs-53097R) react with?
Tested reactivity: Human.
How should bs-53097R be stored?
Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise L3MBTL1 Polyclonal Antibody (bs-53097R)?
Polyclonal antibody raised in rabbit against human L3MBTL1 (lethal 3 malignant brain tumor-like protein), using the N-terminal part of the protein