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L3MBTL1 Polyclonal Antibody

Immunoprecipitation was performed on nuclear extracts from cells transfected with FLAG-tagged L3MBTL1 (L3MBTL1-F) with the Bioss antibody against L3MBTL1 (Cat. No. bs-53097R) and with an IgG negative control antibody. Western Blot was performed with an anti-FLAG antibody to demonstrate the presence of L3MBTL1-F in the input (lane 1), in the precipitated fraction (P) and in the supernatant (SN). These data show that L3MBTL1-F was efficiently precipitated by the L3MBTL1 antibody (lane 3 and 5), whereas it was not precipitated by the IgG negative control antibody (lane 2 and 4).
Immunoprecipitation was performed on nuclear extracts from cells transfected with FLAG-tagged L3MBTL1 (L3MBTL1-F) with the Bioss antibody against L3MBTL1 (Cat. No. bs-53097R) and with an IgG negative control antibody. Western Blot was performed with an anti-FLAG antibody to demonstrate the presence of L3MBTL1-F in the input (lane 1), in the precipitated fraction (P) and in the supernatant (SN). These data show that L3MBTL1-F was efficiently precipitated by the L3MBTL1 antibody (lane 3 and 5), whereas it was not precipitated by the IgG negative control antibody (lane 2 and 4).
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE) using the Bioss antibody against L3MBTL1 (Cat. No. bs-53097R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 2). A molecular weight marker is shown in lane 1; the location of the protein of interest is indicated on the right.
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE) using the Bioss antibody against L3MBTL1 (Cat. No. bs-53097R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 2). A molecular weight marker is shown in lane 1; the location of the protein of interest is indicated on the right.

Applications

  • WB
  • IP
  • IF

Reactivity

  • Human
Overview
Catalog # bs-53097R
Product Name L3MBTL1 Polyclonal Antibody
Applications WB, IP, IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against human L3MBTL1 (lethal 3 malignant brain tumor-like protein), using the N-terminal part of the protein
Clonality Polyclonal
Isotype Not Determined
Concentration 2ug/ul
Purification Whole antiserum from rabbit containing 0.05% azide
Storage Buffer PBS with 0.05% sodium azide and 0.05% Proclin300.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 26013
Swiss Prot Q9Y468
Synonyms Lethal(3)malignant brain tumor-like protein 1, L3MBTL1, KIAA0681, L3MBT, L3MBTL
Background Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
Application Dilution
WB 1:300-5000
IP 1-2ug
IF

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