DNMT3A Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-53128R-50ug |
| Product Name | DNMT3A Polyclonal Antibody |
| Applications |
WB
ELISA
IP
CHIP-SEQ
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58 |
| Clonality | Polyclonal |
| Isotype | Not Determined |
| Concentration | 0.75ug/ul |
| Purification | Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 1788 |
| Swiss Prot | Q9Y6K1 |
| Synonyms | DNMT3A2, TBRS |
| Background | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IP | 1-2ug |
| ChIP-seq | |
Frequently asked questions
Which applications is DNMT3A Polyclonal Antibody (bs-53128R) validated for, and at what dilution?
bs-53128R is validated for WB (1:300-5000); ELISA (1:500-1000); IP (1-2ug); ChIP-seq. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DNMT3A Polyclonal Antibody (bs-53128R) react with?
Tested reactivity: Human.
How should bs-53128R be stored?
Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DNMT3A Polyclonal Antibody (bs-53128R)?
Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58