PML Polyclonal Antibody

Applications

  • WB
  • ELISA

Reactivity

  • Human
Overview
Catalog # bs-53134R
Product Name PML Polyclonal Antibody
Applications WB, ELISA
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against human PML (Promyelocytic leukemia), using 3 different KLH-conjugated synthetic peptides
Clonality Polyclonal
Isotype Not determined
Concentration 0.755ug/ul
Purification Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Storage Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles
Target
Gene ID 5371
Swiss Prot P29590
Synonyms MYL, PP8675, RNF71, TRIM19, PML
Background Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. IsoformPML-4has a multifaceted role in the regulation of apoptosis and growth suppression: activates RB1 and inhibits AKT1 via interactions with PP1 and PP2A phosphatases respectively, negatively affects the PI3K pathway by inhibiting MTOR and activating PTEN, and positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). IsoformPML-4also: acts as a transcriptional repressor of TBX2 during cellular senescence and the repression is dependent on a functional RBL2/E2F4 repressor complex, regulates double-strand break repair in gamma-irradiation-induced DNA damage responses via its interaction with WRN, acts as a negative regulator of telomerase by interacting with TERT, and regulates PER2 nuclear localization and circadian function. IsoformPML-6inhibits specifically the activity of the tetrameric form of PKM. The nuclear isoforms (isoformPML-1, isoformPML-2, isoformPML-3, isoformPML-4and isoform PML-5) in concert with SATB1 are involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. IsoformPML-2is required for efficient IFN-gamma induced MHC II gene transcription via regulation of CIITA. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. PML also regulates transcription activity of ELF4 and can act as an important mediator for TNF-alpha- and IFN-alpha-mediated inhibition of endothelial cell network formation and migration.
Application Dilution
WB 1:300-1000
ELISA 1:500-1000