JMJD2a Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-53136R-50ug |
| Product Name | JMJD2a Polyclonal Antibody |
| Applications |
WB
ELISA
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein |
| Clonality | Polyclonal |
| Isotype | Not Determined |
| Concentration | 0.6ug/ul |
| Purification | Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 9682 |
| Swiss Prot | O75164 |
| Synonyms | JHDM3A, KDM4A, JMJD2, Lysine-specific demethylase 4A |
| Background | Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IF | |
Frequently asked questions
Which applications is JMJD2a Polyclonal Antibody (bs-53136R) validated for, and at what dilution?
bs-53136R is validated for WB (1:300-5000); ELISA (1:500-1000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does JMJD2a Polyclonal Antibody (bs-53136R) react with?
Tested reactivity: Human.
How should bs-53136R be stored?
Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise JMJD2a Polyclonal Antibody (bs-53136R)?
Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein