phospho-CD95/FAS (Tyr291) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5323R-Cy3 |
| Product Name | phospho-CD95/FAS (Tyr291) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FAS around the phosphorylation site of Tyr291 |
| Modification Site | Tyr291 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 355 |
| Swiss Prot | P25445 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; FAS |
| Background | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CD95/FAS (Tyr291) Polyclonal Antibody, Cy3 Conjugated (bs-5323R-Cy3) validated for, and at what dilution?
bs-5323R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CD95/FAS (Tyr291) Polyclonal Antibody, Cy3 Conjugated (bs-5323R-Cy3) react with?
Tested reactivity: Human.
How should bs-5323R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CD95/FAS (Tyr291) Polyclonal Antibody, Cy3 Conjugated (bs-5323R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human FAS around the phosphorylation site of Tyr291
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5323R-Cy3)?
Cy3 on bs-5323R-Cy3: excitation / emission 512,550nm/570,615nm.